In Vitro Regeneration of selected local Tomato Cultivars in Palestine

Year: 
2013
Discussion Committee: 
Dr. Hassan Abu Qaoud/supervisor
Dr. Rami Arafeh /External Examiner
Dr. Raed Alkowni /Internal Examiner
Supervisors: 
Dr. Hassan Abu Qaoud/supervisor
Authors: 
Fedaa Hazem Jamous
Abstract: 
A study was conducted to investigate the effect of different growth factors on shoot multiplication and regeneration on some tomato cultivars planted in Palestine. Cultivar 593 and Baladi landrace were involved in these experiments with four explants (hypocotyl, cotyledon, stem, and leaf). The effect of different levels and combinations of auxin Naphthalene acetic acid (NAA) and cytokinin Benzyl adenine (BA), Kinetin (Kin) and N-1,2,3-Thiadiazol-5-yl-N’-phenylurea (TDZ) on shoot multiplication and regeneration was examined in this study. Shoot tips from established seedlings were used as source materials for shoot multiplication. The shoot tips were cultured on Murashige and Skoog media (MS medium) with (2.2, 4.4 µM) BA, (9.2, 18.4 µM) Kinetin combined with 0.0, 2.7 µM NAA. The higher shoot number (8.4) was obtained with MS medium supplied with 18.4 µM Kinetin. Low shoots number were observed on MS medium supplied with 18.4 µM Kinetin and 2.7 µM NAA in Baladi cultivar. Direct regeneration with four different explants was obtained on MS basal medium supplemented with TDZ at (0.0, 1.0, 2.0 and 4.0 µM) and NAA at (0.0, 2.7 and 5.4 µM), as well as BA at (0.0 or 2.2 µM) and Kinetin at (0.0, 2.3 µM) in two separated experiments. The highest shoot percentage (62.25%) was obtained with Kinetin and BA at (2.3 and 2.2 µM) respectively. However, when NAA and TDZ were combined, 46.9% shoot regeneration was achieved with 2.7 and 4.0 µM, respectively. Very low shoot regeneration was observed with all NAA levels combined with 1.0 and 2.0 µM TDZ. High rooting percentage (100%) was obtained when NAA was added to the media at (2.7 and 5.4 µM).
Full Text: 
Pages Count: 
68
Status: 
Published