In Vitro Regeneration of Local Chickpea Varieties in Palestine

Discussion Committee: 
Dr. Hassan Abu Qaoud/supervisor
Dr. Rami Arafeh /External Examiner
Dr. Heba Al-Fares /Internal Examiner
Dr. Hassan Abu Qaoud/supervisor
Rozan Irfan Al-Tanbouz
A study was conducted to investigate the effect of different factors on shoot multiplication and regeneration of chickpea (Cicer arietinum L.) Three varieties (HUDAS, FLIP03-147c and FLIP05-100C) and two Palestinian landraces 'Ein Al Bayda' and 'Baladi' were used in this study. The effect of different levels and combinations of the auxin 1-Naphthaleneacetic acid (NAA) and the cytokinins Benzyl adenine (BA), Kinetin (Kin) and N-1, 2, 3-Thiadiazol-5-yl-N’-phenylurea (TDZ) on shoot multiplication and regeneration was examined in thisstudy. Shoots tip from in vitro established seedlings were used as a source material for shoot multiplication. The shoot tips were cultured on MS medium supplied with 0.0, 2.2 and 4.4 µM BA, or 0.0, 9.2 and 18.4 µM Kinetin, both cytokinins were combined with 0.0 or 2.7 µM NAA. The highest shoot number (8.0) was obtained on media without hormone or media supplied with 2.2 µM BA in the Baladi landrace. However, Low shoots number wereobserved on MS medium supplied with 9.2 or 18.4 µM Kin and 2.7 µM NAA in both landraces. For shoot regeneration stem and leaf explants of the studied cultivars and landraces were cultured on MS media supplied with different combinations of TDZ, Kinetin and NAA. The highest shoot percentage (62.0%) and number of shoots (3.0) was obtained with leaf explant using 2.0 μM TDZ with ‘FLIP05-100C’, stem explants exhibited no shoot regeneration in all five chickpea varieties. Very low shoot regeneration was observed with all NAA level combined with TDZ. However, regeneration percentage was highly reduced with higher cytokinin levels. No shoot regeneration was observed with any Kinetin level.
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