Evaluation of Imidacloprid and Abamectin Residues in Tomato, Cucumber and Pepper by High Performance Liquid Chromatography (HPLC)

Discussion Committee: 
Dr. Shehdeh Jodeh / ( Supervisor )
Prof. Marwan Haddad / ( Co – Supervisor )
Dr. Wadie Sultan/ ( External Examiner )
Dr. Hassan Abu Qaoud / ( Internal Examiner )
Dr. Shehdeh Jodeh
Saed Mousa Diab Ali
Recently, one of the most indispensable methods is the usage of Imidacloprid (Konfedor® ) and Abamectin ( Vertimk® ) in crops which make great improvement toward this crops such as tomato, cucumber and pepper. On the other hand there are demerits of using such pesticides toward the human health. In this study, we choose two types of pesticides which mentioned above (Imidacloprid and Abamectin), for many reasons, first of all they are the most common among farmers as shown in questioner, second, this pesticides are the most effective in coming over the spiders and many insects. Imidacloprid (Konfedor® ) and abamectin ( Vertimk® ) where sprayed on tomato, cucumber and pepper in three greenhouses using the same concentration that farmers used to spray their crops, concentration of imidacloprid (Konfedor® ) was 14.5mg /L and concentration of abamectin ( Vertimk® ) was 29 mg /L. Samples from different crops and soil were collected of spraying , five days, ten days and twenty days later. Samples were taken from fruits, leaves and roots. After collection of samples they were extracted. Then the extracts were analyzed by high performance liquid chromatography ( HPLC ) and ultra violet visible spectrophotometer to evaluate the residues of imidacloprid (Konfedor® ) and abamectin ( Vertimk® ). Results obtained from this study indicates that the residues of both imidacloprid (Konfedor® ) and abamectin ( Vertimk® ) were higher than the quantities of residues that determined by previous researchers. Also results obtained from this study showed that the quantities of residues were higher than the maximum residue levels ( MRLs ) in the samples that are collected on the first, the fifth and the tenth day of spraying. Also this study showed that the residues of abamectin are higher than imidacloprid, and residues of both abamectin and imidacloprid are higher in soil than plant parts.
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