Sanitary assessment of olive varieties in a collection plot at the University of Perugia (Italy)

Osama Al-abdallah
Elbeaino, T
Farinelli, D
Valentini, F
Digiaro, M

A survey was carried out in an olive collection plot at the University of Perugia (Umbria,Italy), for assessing the sanitary status of a representative olive plants were selected fromdifferent local and international olive trees varieties in order to evaluate the possibility toutilize the selected olive plants maintained in the collection plot at University of Perugia aspotential mother plants to multiply in the framework of the olive certification program. Oliveis affected by a number of virus and virus-like agents that persist in the plant material withwhich they can be transmitted and disseminated (Martelli et al., 2002). Their presence in theplant is sometimes associated with symptoms, but more often infections are latent (AlkowniR. 2000). Cardoso et al., 2005).A total of 95 olive samples were tested by RT-PCR to check for the presence of Arabismosaic virus (Ar-MV), Cherry leaf roll virus (CLRV), Cucumber mosaic virus (CMV), Oliveleaf yellowing associated virus (OLYaV), Olive latent ringspot virus (OLRSV), Olive latentvirus-1 (OLV-1), Olive latent virus-2 (OLV-2), and Strawberry latent ringspot virus(SLRSV). 0.2g from olive phloem tissues were subjected for extraction of total nucleic acids,then cDNA was synthesized by Moloney Murine Leukaemia virus (M-MLV) reversetranscriptase using random hexamers primer. cDNA mixture were submitted to PCRamplification using specific primers for 8 viruses were previously mentioned (Alabdullah al 2005). DsRNA and mechanical transmission tests were applied to all PCR-negativesamples in order to detect other viruses different from those were detected by PCR (Dodds,1993).About 83% of plants were infected by at least one virus. All tested viruses were present, withthe prevalence of CMV and OLRSV (more than 30% of infection). Eight out of 17 samplesshowed different dsRNA patterns, thus suggesting the presence of infection by viruses otherthan those previously checked by PCR. This study allowed identifying 9 virus free and 39virus tested candidate clones potentially useful as mother plants to be used in certificationprogrammes.Nevertheless, in order to be recognized as primary sources and for satisfying the sanitaryrequirements of the national certification protocol, these candidate clones need to be checkedfor the absence of other graft-transmissible diseases included in certification scheme.