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The study population consisted of 237 children aged 5-6 years in Jenin city. The arterial blood pressure (systolic and diastolic), heart pulse rate, oxygen saturation in blood and tympanic temperature were measured before and after exposure to light intensity levels for four hours continuously. The light intensity levels were (0 - 60), (400 - 600) and (1320 - 1500) lux. The background light was (400 - 600) lux for all selected schools, which is considered to be normal or accepted light intensity level. Strong positive correlation (Pearson Correlation Coefficient) was found to be R > 0.659 between light intensity levels and all of the arterial blood pressure (systolic and diastolic), heart pulse rate, blood oxygen saturation and tympanic temperature in the selected school. In addition, P-value between the dependent and independent variables was found to be zero. Finally, the study concludes that there is an effect of the light intensity levels on arterial blood pressure (systolic and diastolic), heart pulse rate, oxygen saturation in blood and tympanic temperature of schools’ children.