Phytochemical Investigation of antimicrobial Seed Extrac of Citrus Aurantifolia (Lime)

The seeds of Lime of the family Ructaceae are gaining grounds as important source fortreatment in complementary medicine. The Sudanese varieties are one of the best in themarket, which prompted investigation of seed extracts.The 96% ethanolic extract exhibited significant antimicrobial activity and highlighted thebiological monitoring of activity in order to isolate the active metabolites from the chloroformextract of the seeds.The presence of sterols and triterpenes, carotenoids, coumarins, alkaloids, saponins, tanninsand carbohydrates was confirmed by phytochemical screening of the diethyl ether, methanolicand aqueous extracts of the seeds. Isolation of the antibacterial secondary metabolites wasachieved by fractionation of the active chloroform extract by sing, liquid solid columnchromatographic technique and biological monitoring of activity of column fractions elutedwith chloroform and methanol. The composition of fractions was monitored by analytical andpreparative TLC from lime extracts we prepare an ointments with different concentrations.